|The Genetics Laboratory has acquired a Fluidigm EP 1
Washington Department of Fish and Wildlife Molecular Genetics
Laboratory (WDFW-MGL) has been operating since 1985, first operating
as the Department of Fisheries, Genetic Stock Identification
Laboratory, under the direction of Dr. James Shaklee, and focusing
almost entirely on stock identification and fishery analyses.
In 1997, the laboratory operations expanded to include analyses
of DNA sequences and microsatellites, and in 2005 to include
SNP markers. In late 2005, Dr. Shaklee retired, and the Fish
Genetic Laboratory merged with the Wildlife Conservation Genetics
program to form the current Molecular Genetics Laboratory, under
the direction of Dr. Kenneth Warheit (formerly with WDFW Wildlife
Conservation Genetics program).
WDFW-MGL employs a staff of 10 full-time employees, and processes
roughly 20-30,000 samples per year using primarily microsatellite,
sequence, and SNP markers. During the past three years, laboratory
personnel have completed or will complete over 120 fish-related
projects, with financial support from the US Federal Government
(e.g., Pacific Salmon Commission, Bonneville Power Authority
(BPA), Bureau of Land Management (BLM), State Wildlife Grants
(SWG), and through the Anadromous Fish Act (PL-304) or Mitchell
Act), Washington State (General Fund), county Public Utility
Districts (PUDs – dam operators), Native American Tribes,
In addition to being one of the principal genetics laboratories
contributing to the standardization of allozyme, microsatellite,
and SNP markers in Chinook, chum, and coho salmon, steelhead,
and bull trout, WDFW-MGL is also a major repository of fish
tissue samples (for DNA analysis), with 2500 individual collections
incorporating a minimum of 375,000 individual fish, representing
over 50 fish species.
Department of Fish and Wildlife Molecular Genetics Laboratory
is located in Olympia, Washington, and operates a state-of-the-art
molecular laboratory. The lab is constructed as a three-room
concourse with separate DNA extraction, PCR, and freezer rooms/sections.
The total size of the lab is 2700 sq ft, not including individual
office space for technicians, biologists, and research scientists
and an off-site storage facility. See Labworks section for
a list of primary instruments operated by the laboratory.